MICROBIOLOGICAL HYDROXYLATION OF STEROIDS

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The microbiological hydroxylation of steroids and related compounds.

The factors which determine the specific positions at which steroids are hydroxylated by micro-organisms are being investigated, using mono-ketones or keto-alcohols of the androstane series as substrates and infra-red and n.m.r. spectroscopy to determine the structures of the products. Whereas the major product of the hydroxylation of 5o-androstan-3-one by cultures of Calonectria decora is 123,...

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The Microbiological Degradation of Steroids

Belin, P. (1926). Bull. Soc. Chim. biol., Paris, 8, 1081. Bernheim, F. (1942). J. Bact. 44, 533. Birkinshaw, J. H. & Raistrick, H. (1931). Philos. Tras. B, 220, 11. Bloor, W. R. (1929). J. biol. Chem. 82, 273. den Dooren de Jongh, L. E. (1926). Ned. Tijdschr. Hyg. 1, 136. Fink, H., Haehn, H. & Hoerburger, W. (1937). Cher. Ztg, 61, 689, 723, 744.. Fiske, C. H. & Subbarow, Y. (1925). J. biol.' Ch...

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Continuous microbiological transformation of steroids.

Continuous fermentation trials on the bioconversions of pregnadiene to pregnatriene by Septomyxa affinis and progesterone to 11alpha-hydroxyprogesterone by Rhizopus nigricans were conducted successfully in an eight-stage pilot plant reactor. The first stage was used as the mycelial growth stage while the steroid solutions were added continuously to stage 2, thus using the remaining stages as co...

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Microbiological hydroxylation of cinerone to cinerolone.

Cinerone [2-(2'-cis-butenyl)-3-methyl-2-cyclopenten-1-one] is hydroxylated to cinerolone [2-(2'-cis-butenyl)-3-methyl-4-hydroxy-2-cyclopenten-1-one] by a number of streptomycetes, bacteria, and fungi. Aspergillus niger ATCC 9,142 and Streptomyces aureofaciens ATCC 10,762 were found to be the most effective hydroxylators. The optical activity of the product was found to range from [alpha](D) (25...

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Hydroxylation of steroids at carbon 21.

Hydroxylation of steroids in V&O at carbon atom 21 by adrenal tissue preparations has previously been reported by Plager and Samuels (l), Hayano and Dorfman (2), and the present authors (3). The former workers described a system consisting of a 20,000 X Q supernatant fraction of beef adrenals which effected hydroxylation in this position of progesterone and ita derivatives in the presence of ad...

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ژورنال

عنوان ژورنال: The Journal of General and Applied Microbiology

سال: 1958

ISSN: 1349-8037,0022-1260

DOI: 10.2323/jgam.4.79